Cypex CYP2C18 QC Assays
buffer: 0.05 M potassium phosphate pH 7.4, 5 mM MgCl2
volume: 200 µl
Stop reagent: methanol (200 µl)
Column: Hypersil ODS (5 µm) 250 x 4 mm
Mobile phase: 20 mM potassium phosphate pH 7/methanol/acetonitrile
60/22.5/17.5 (v/v/v) (isocratic)
Flow rate: 1.0 ml/min
Run time: 13.5
Injection volume: 100 µl
Detection: UV, l
= 280 nm
is available from Cypex Ltd
of phosphate concentration on diclofenac turnover by CYP2C18LR Bactosomes
4'-hydroxylase activity is inhibited at phosphate concentrations above 50 mM.
assay was carried out at 200 µM diclofenac at a P450 concentration of 5
pmol per 200 µl.
under licence from BTG International Ltd (AU730155, EP0914446, US6566108 and other
United States Patent Nos. 5,420,027 or 5,240,831, Canada
Patent No. 2100245 and other patents pending.