Cypex CYP2C8 QC Assays
buffer: 0.1 M potassium phosphate pH 7.4, 5 mM MgCl2
volume: 200 µl
Stop reagent: acetonitrile (100 µl)
Column: Hypersil BDS-C18 (3 µm) 100 x 4 mm
Mobile phase: 20 mM potassium phosphate pH 7/acetonitrile 57.5/42.5
Flow rate: 1.0 ml/min
Run time: 8.5 min
volume: 75 µl
Detection: UV, l
= 230 nm
substrate, paclitaxel, and metabolite,
6alpha-hydroxypaclitaxel are both available
from Cypex Ltd.
of phosphate concentration on paclitaxel turnover by CYP2C8R Bactosomes
by CYP2C8 is sensitive to the concentration of phosphate in the incubation.
assay was carried out at 25 µM paclitaxel at a P450 concentration of 2 pmol
per 200 µl.
under licence from BTG International Ltd (AU730155, EP0914446, US6566108 and other
United States Patent Nos. 5,420,027 or 5,240,831, Canada
Patent No. 2100245 and other patents pending.