Cypex CYP2J2 QC Assays

Activity: Terfenadine C-hydroxylase

Incubation conditions
Assay buffer: 0.1 M potassium phosphate pH 7.4, 5 mM MgCl₂
Incubation volume: 200 µl
Stop reagent: 1 M hydrochloric acid (25 µl)

HPLC conditions
Column: Gemini C18 (3 µm) 100 x 4.6 mm
Temperature: 45°C
Mobile phase: Water/0.5% trifluoroacetic acid/acetonitrile 42/20/38 (v/v/v) initial (gradient separation)
Flow rate: 1.0 ml/min
Run time: 10.0 min
Injection volume: 100 µl
Detection: fluorescence l(ex) = 214 nm, l(em) = 310 nm
Retention times:
       terfenadine alcohol         3.2 min

The CYP2J2 substrate terfenadine is available from Cypex. 

Made under licence from BTG International Ltd (AU730155, EP0914446, US6566108 and other patents pending).

United States Patent Nos. 5,420,027 or 5,240,831, Canada Patent No. 2100245 and other patents pending.