Cypex CYP4A11 QC Assays

Activity: Lauric acid 12-hydroxylase

Incubation conditions
Assay buffer: 0.1 M Tris-HCl pH 7.4, 5 mM MgCl₂
Incubation volume: 100 µl
Stop reagent: acetonitrile/acetic acid, 98/2 (v/v) (50 µl)
Internal standard: not used

HPLC conditions
Column: HyperClone ODS (3 µm) 150 x 3.2 mm
Temperature: 40°C
Mobile phase: 0.05% acetic acid/acetonitrile, 65/35 (v/v) initial (gradient separation)
Flow rate: 0.6 ml/min
Run time: 17.5 min
Injection volume: 100 µl
Detection: radiometric
Retention times:
     12-hydroxylauric acid      7.6 min
     lauric acid                     14.5 min

The substrate, lauric acid and the metabolites 11-hydroxylauric acid and 12-hydroxylauric acid are available from Cypex Ltd

Made under licence from BTG International Ltd (AU730155, EP0914446, US6566108 and other patents pending).

United States Patent Nos. 5,420,027 or 5,240,831, Canada Patent No. 2100245 and other patents pending.