Cypex ALDH1A1 QC Assay

Activity: 4-nitrobenzaldehyde oxidase

Incubation conditions
Assay buffer: 0.05 M Hepes pH 7.4, 1 mM EDTA, 1 mM NAD⁺
Incubation volume: 500 µl
Stop reagent: 1 M hydrochloric acid (62.5 µl)

HPLC conditions
Column: HyperClone BDS-C18 (3 µm) 125 x 4 mm
Temperature: 30°C
Mobile phase: 0.5% trifluoroacetic acid/acetonitrile/methanol 55/10/35 (v/v/v) (isocratic)
Flow rate: 1.0 ml/min
Run time: 5.0 min
Injection volume: 100 µl
Detection: UV, l = 264 nm
Retention times:
       4-nitrobenzaldehyde      2.7 min
       4-nitrobenzoic acid        3.3 min

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United States Patent Nos. 4,952,496, 5,693,489 and 5,869,320.