Cypex aldehyde oxidase QC Assay

Activity: phthalazine oxidase

Incubation conditions
Assay buffer: 0.025 M potassium phosphate pH 7.4, 0.1 mM EDTA
Incubation volume: 100 µl
Stop reagent: 1 M hydrochloric acid (12.5 µl)

HPLC conditions
Column: Gemini C18 (3 µm) 100 x 4.6 mm
Temperature: 20°C
Mobile phase: 0.05% (v/v) orthophosphoric acid/acetonitrile; 85/15 (v/v) (isocratic)
Flow rate: 1.0 ml/min
Run time: 7.0 min
Injection volume: 50 µl
Detection: fluorescence l(ex) = 285 nm, l(em) = 340 nm
Retention times:
       phthalazine         1.6 min
     1-phthalazinone       4.5 min

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