Cypex CES QC Assay

Activity: 4-nitrophenyl butyrate hydrolysis

Note: This activity is measured using a continuous spectrophotometric procedure in a plate reader.

Incubation conditions
Assay buffer: 0.05 M potassium phosphate pH 7.4/acetonitrile 98/2 (v/v)
Incubation volume: 0.25 ml
Incubation temperature: 30ºC
Detection wavelength: 400 nm

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