Human CYP1A2LR Bactosomes

Catalogue Number: CYP012

Product Description: Human CYP1A2 and human CYP-reductase coexpressed in Escherichia coli

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Note: This product is also available in our convenient EasyCYP format.

Typical Batch Characteristics

P450 Concentration: 6 nmol/ml

Protein Concentration: 7.5 mg/ml

Specific P450 Content:
      800 pmol/mg protein

Cytochrome c Reductase Activity:
      150 nmol/min/mg protein

Typical P450 Activity Data (1)

Activity: 7-ethoxycoumarin O-deethylase

Linear with time up to: 20 min

Linear with P450 up to: 50 pmol/ml

Vmax: 0.1 pmol/min/pmol CYP

Km: 1.9 µM

Typical P450 Activity Data (2)

Activity: 7-ethoxyresorufin O-deethylase

Linear with time up to: 6 min

Linear with P450 up to: 40 pmol/ml

Vmax: 1.1 pmol/min/pmol CYP

Km: 0.3 µM

Note: Incubation time 30s for P450 linearity test. Formation of resorufin deviates significantly from linearity at P450 concentrations less than 5 pmol/ml.

Product Information Sheets

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Made under licence from BTG International Ltd (AU730155, EP0914446, US6566108 and other patents pending).

United States Patent Nos. 5,420,027 or 5,240,831, Canada Patent No. 2100245 and other patents pending.