Human CYP1B1LR EasyCYP Bactosomes

Catalogue Number: CYP/EZ023

Product Description: Human CYP1B1.1 and human CYP-reductase coexpressed in Escherichia coli

Amount: 0.5 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Typical Batch Characteristics

P450 Concentration: 1 nmol/ml

Protein Concentration: 10 mg/ml

Specific P450 Content:
     100 pmol/mg protein

Cytochrome c Reductase Activity:
      40 nmol/min/mg protein

Typical P450 Activity Data (1)

Activity: 17b-estradiol 4-hydroxylase

Linear with time up to: 20 min

Linear with P450 up to: 50 pmol/ml

Vmax: 1.0 pmol/min/pmol CYP

Km: 0.5 µM

Typical P450 Activity Data (2)

Activity: 7-ethoxyresorufin O-deethylase

Linear with time up to: 2 min

Linear with P450 up to: 20 pmol/ml

Vmax: 6 pmol/min/pmol CYP

Km: 0.1 µM

Note: Incubation time 10s for P450 linearity test. Formation of resorufin deviates significantly from linearity at P450 concentrations less than 2 pmol/ml.

Product Information Sheets

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Made under licence from BTG International Ltd (AU730155, EP0914446, US6566108 and other patents pending).

United States Patent Nos. 5,420,027 or 5,240,831, Canada Patent No. 2100245 and other patents pending.