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Human
CYP2C8BR Bactosomes
Catalogue
Number:
CYP049
Product
Description:
Human CYP2C8 and human CYP-reductase coexpressed in Escherichia coli
and supplemented with purified human cytochrome b5
Amount:
1 nmol cytochrome P450
Storage
Buffer:
50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage:
Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended
Use: For laboratory (research) purposes only
Note:
This product is also available in our convenient EasyCYP
format.
Typical
Batch Characteristics
P450
Concentration:
6 nmol/ml
Protein
Concentration:
12 mg/ml
Cytochrome
b5
Concentration:
30 nmol/ml
Specific
P450 Content:
500 pmol/mg
protein
Cytochrome
c Reductase Activity:
800
nmol/min/mg protein
Typical
P450 Activity Data (1)
Activity:
Paclitaxel 6a-hydroxylase
Linear
with time up to:
6 min
Linear
with P450 up to:
40 pmol/ml
Vmax:
5 pmol/min/pmol CYP
Km:
4 µM
Typical
P450 Activity Data (2)
Activity:
Tolbutamide 4-hydroxylase
Linear
with time up to:
12 min
Linear
with P450 up to:
100
pmol/ml
Vmax:
3 pmol/min/pmol CYP
Km:
1100 µM (see note below)
Note:The
kinetic parameters given above were determined using tolbutamide
concentrations from 40 - 500 µM, as the limit of solubility
is reported to be around 740 µM. The margin of error is
therefore likely to be high. The tolbutamide 4-hydroxylase activity of
CYP2C8BR
Bactosomes at 500 µM tolbutamide is approximately 1.2
pmol/min/pmol CYP.
Product
Information Sheets
More
Information Available
Important:
By purchasing this product from Cypex you accept the standard terms and conditions
of supply, details of which may be provided upon request.
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Made
under licence from BTG International Ltd (AU730155, EP0914446,
US6566108 and other patents pending).
United States Patent Nos. 5,420,027 or 5,240,831, Canada Patent No.
2100245 and other patents pending.
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