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Human
CYP4F3BBLR Bactosomes
Catalogue
Number:
CYP057
Product
Description:
Human CYP4F3B and human CYP-reductase coexpressed in Escherichia coli
and supplemented with purified human cytochrome b5
Amount:
1 nmol cytochrome P450
Storage
Buffer:
50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage:
Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended
Use: For laboratory (research) purposes only
Note:
This product is also available in our convenient EasyCYP
format.
Typical
Batch Characteristics
P450
Concentration:
3 nmol/ml
Protein
Concentration:
15 mg/ml
Cytochrome
b5
Concentration:
15
nmol/ml
Specific
P450 Content:
200 pmol/mg
protein
Cytochrome
c Reductase Activity:
200
nmol/min/mg protein
Typical
P450 Activity Data
Activity:
Leukotriene B4
omega-hydroxylase
Linear
with time up to:
20 min
Linear
with P450 up to:
120
pmol/ml
Vmax:
0.8
pmol/min/pmol CYP
Km:
2 µM (see note below)
Note:
The Km for CYP4F3B expressed in E.
coli is
approximately 10-fold lower
than that reported for CYP4F3B expressed in insect cells. The reason
for this is unknown.
Product
Information Sheets
More
Information Available
Important:
By purchasing this product from Cypex you accept the standard terms and conditions
of supply, details of which may be provided upon request.
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Made
under licence from BTG International Ltd (AU730155, EP0914446,
US6566108 and other patents pending).
United States Patent Nos. 5,420,027 or 5,240,831, Canada Patent No.
2100245 and other patents pending.
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