Cypex Plate Reader Assays

Introduction
The following pages give details of protocols for determining cytochrome P450 activity using a fluorescence plate reader. The optimal incubation conditions can also be used to investigate CYP inhibition in a high-throughput format.

CYP1A2 (7ER)

CYP2C9 (MFC) (updated 13th March 2008)

CYP2C19 (OMF)

CYP2D6 (MAMC)

CYP3A4 (7BQ) (updated 20th March 2008)

The instrument used at Cypex is a Tecan Infinite M-200 equipped with two dispensers.

The non-plate reader QC assays used at Cypex can be found on this page.

Made under licence from BTG International Ltd (AU730155, EP0914446, US6566108 and other patents pending).

United States Patent Nos. 5,420,027 or 5,240,831, Canada Patent No. 2100245 and other patents pending.