Polyclonal antiserum raised in goat against purified rat
200 µl serum
Store at -20°C. Avoid frequent temperature changes. Thaw on ice.
Use: For laboratory (research) purposes only.
Immunoinhibition and Western blotting.
of immunoinhibition against rat CYPs:
***: strong inhibition observed, **: moderate
inhibition observed, *: weak inhibition observed,
-: no inhibition observed
showing immunoinhibition of 7-ethoxycoumarin O-deethylation in liver
microsomes prepared from a 3-methylcholanthrene-treated rat. Anti-serum
(0 - 50 µl) was pre-incubated with rat liver microsomes
(200 µg protein) at room temperature for 30 min
before starting the reaction. The final incubation volume was
500 µl. Data published by Daiichi Pure Chemical Co. Ltd.
Western blot of liver microsomes prepared from a 3-methylcholanthrene
treated rat (0.3 µg protein), showing the specificity of the
R-PAP041 antibody. The band labelled '1' is reported to represent
CYP2E1. Data published by Daiichi Pure Chemical Co. Ltd.
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