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Cypex SULT1A1*1 QC Assays Activity: 4-nitrophenol
sulfotransferase Incubation
conditions
Assay
buffer: 0.05 M potassium phosphate pH 7.4, 5 mM MgCl2, 10 mM dithiothreitol
Incubation volume: 200 µl
Stop reagent: acetonitrile (100 µl) HPLC
conditions
Column:
Hypersil ODS (5 µm) 250 x 4 mm
Temperature: 30°C
Mobile phase: 5%
triethylamine (pH to 3 with H3PO4)/acetonitrile 66/34 (v/v)
(isocratic)
Flow rate: 1.0 ml/min
Run time: 10.0 min
Injection volume:
100 µl
Detection: UV, l = 280 nm
Retention
times:
4-nitrophenylsulfate 4.2
min
4-nitrophenol 5.2
min Activity: 4-methylumbelliferone
sulfotransferase Incubation
conditions
Assay
buffer: 0.05 M potassium phosphate pH 7.4, 5 mM MgCl2, 10 mM dithiothreitol
Incubation volume: 200 µl
Stop reagent: acetonitrile (100 µl) HPLC
conditions
Column:
Hypersil ODS (5 µm) 250 x 4 mm
Temperature: 30°C
Mobile phase: 50
mM ammonium acetate, 50 mM tetrabutylammonium (pH 3.6)/
acetonitrile;
54/46 (v/v) (isocratic)
Flow rate: 1.0 ml/min
Run time: 7.0 min
Injection
volume: 100 µl
Detection: fluorescence l(ex) = 315 nm, l(em) = 375 nm
Retention
times:
4-methylumbelliferone 3.3
min
4-methylumbelliferylsulfate 4.3
min Important: By purchasing this
product from Cypex you accept the standard terms and conditions of supply, details of which may
be provided upon request. United
States Patent Nos. 4,952,496, 5,693,489 and 5,869,320. | 
