Cypex SULT1C2 QC Assay

Activity: p-nitrophenol sulfotransferase

Incubation conditions
Assay buffer: 0.05 M potassium phosphate pH 7.4, 5 mM MgCl₂, 10 mM dithiothreitol
Incubation volume: 200 µl
Stop reagent: acetonitrile (100 µl)

HPLC conditions
Column: Hypersil ODS (5 µm) 250 x 4 mm
Temperature: 30°C
Mobile phase: 5% (v/v) triethylamine (pH 3.0)/acetonitrile; 66/34 (v/v) (isocratic)
Flow rate: 1.0 ml/min
Run time: 10.0 min
Injection volume: 100 µl
Detection: UV, l = 280 nm
Retention times:
      p-nitrophenylsulfate      4.2 min
      p-nitrophenol               5.2 min

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United States Patent Nos. 4,952,496, 5,693,489 and 5,869,320.