Cypex SULT1C4 QC Assay

Activity: 4-methylumbelliferone sulfotransferase

Incubation conditions
Assay buffer: 0.05 M potassium phosphate pH 7.4, 5 mM MgCl₂, 10 mM dithiothreitol
Incubation volume: 200 µl
Stop reagent: acetonitrile (100 µl)

HPLC conditions
Column: Hypersil ODS (5 µm) 250 x 4 mm
Temperature: 30°C
Mobile phase: 50 mM ammonium acetate, 50 mM tetrabutylammonium (pH 3.6)/
     acetonitrile; 54/46 (v/v) (isocratic)
Flow rate: 1.0 ml/min
Run time: 7.0 min
Injection volume: 100 µl
Detection: fluorescence l(ex) = 315 nm, l(em) = 375 nm
Retention times:
       4-methylumbelliferone              3.3 min
     4-methylumbelliferylsulfate        4.3 min

Important: By purchasing this product from Cypex you accept the standard terms and conditions of supply, details of which may be provided upon request.

United States Patent Nos. 4,952,496, 5,693,489 and 5,869,320.