|
Cypex UGT1A6 QC Assays Substrate: 1-naphthol Incubation
conditions
Assay
buffer: 0.05 M potassium phosphate pH 7.4, 10 mM MgCl2, 5 mM saccharolactone
Incubation volume: 200 µl
Stop reagent: acetonitrile (100 µl) HPLC
conditions
Column:
Hypersil ODS (5 µm) 250 x 4 mm
Temperature: 30°C
Mobile phase: 0.05%
orthophosphoric acid/acetonitrile (gradient separation)
Flow rate: 1.0 ml/min
Run time: 14.5 min
Injection volume: 100 µl
Detection: fluorescence
l(ex) = 280 nm, l(em) = 335 nm
Retention
times:
1-naphthylglucuronide 5.2
min
1-naphthol 11
min Substrate: 4-methylumbelliferone
(4-MU) Incubation
conditions
Assay
buffer: 0.05 M potassium phosphate pH 7.4, 10 mM MgCl2, 5 mM saccharolactone
Incubation volume: 200 µl
Stop reagent: 1 M hydrochloric acid (20 µl) HPLC
conditions
Column:
Hypersil ODS (5 µm) 250 x 4 mm
Temperature: 30°C
Mobile phase: 0.05%
orthophosphoric acid/acetonitrile (gradient separation)
Flow rate: 1.0 ml/min
Run time: 15.0 min
Injection volume: 10 µl
Detection: fluorescence
l(ex) = 315 nm, l(em) = 375 nm
Retention
times:
4-MU
glucuronide 3.8
min
4-methylumbelliferone 8.9
min Important: By purchasing this
product from Cypex you accept the standard terms and conditions of supply, details of which may
be provided upon request. United
States Patent Nos. 4,952,496, 5,693,489 and 5,869,320. | 
